The global mailing list where everything diybio is discussed:
10 most recent discussion topics:
- PCR thermocyler concepts - old designsDiscovered some old images where I was playing with thermocycler ideas. First one is based on a hair dryer - my take the record for the slowest ramp rates! Second is practical - based on a straight halogen lamp and a cylindrical tube.
- SHA2017No plans for the summer yet? Sign up for SHA2017! https://sha2017.org/call-for-participation Although the main focus is digital hacking and security, any type of hacking is represented at these camps. Biology is rapidly becoming digital anyway. So let's create a biohack camp too. For example,
- Simple His-tag purificationA friend and I were talking about a simplified His-tag purification protocol for a peptide we'd like to produce and use, and I wanted to see what other people thought of it's feasibility. Instead of applying the cell lysate to a nickel column, first add nickel powder ($5 on ebay) to the lysate
- Fusion protein softwareDoes anyone know of any decent software for modelling/ constructing fusion proteins, starting with the DNA sequences? The LINKER program seems to be defunct,and doing it "by hand " is tedious.
- Didatic ways for explication about Action potencial:Hello, My name is Danilo, I am an student of science licentiate and I search experiments about action potencial for Middle School . What's yours suggest? Thank you, Danilo.
- Odin's Blessings for Friday $75 PCR Machine, $30 Pipettes and a bunch of StrainsIt's Friday and time for another installment of Odin's blessings. Use the links to buy the items on our site. If it won't let you purchase it that means it is out of stock and someone beat you to it. This week we have a 96 well MJ Research PTC-100 Thermal cycler for $75!! Free shipping to
- Brian Hanley's garage gene therapyHey guys. I came across this story https://www.technologyreview.com/s/603217/one-mans-quest-to-hack-his-own-genes/?utm_campaign=internal&utm_medium=homepage&utm_source=cover-story It seems that somebody injected himself with plasmid DNA and electroporated it then. His goal was to express human
- Stanford bioengineers develop a 20-cent, hand-powered centrifugeThis is probably as simple as a centrifuge can get. Can this design be improved? Can features be added? https://youtu.be/pPePaKnYh2I "Inspired by a whirligig toy, Stanford bioengineers have developed an ultra-low-cost, human-powered blood centrifuge. With rotational speeds of up to 125,000
- Necessity of Carrier DNA?Hello DIY Bio Community! My name is Han. I am a student who's been interested in DIY Bio, and I wanted to undergo my own project. I have recently been following the JHU 2011 VitaYeast IGEM project (http://2011.igem.org/Team:Johns_Hopkins) in an attempt to recreate their yeast transformation as
- Adding Phenol Red to plant tissue culture media?Hello, after doing some reading on the effects of autoclaving and callus to TC agar media pH, I've come to the conclusion that the actual pH can vary quite wildly with time compared to the measured pH prior to autoclaving. I was thinking if an indicator like phenol red can be added to the media